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Results: 26
Direct digital sensing of protein biomarkers in solution.
Georg Krainer, Kadi L Saar, William E Arter, Timothy J Welsh, Magdalena A Czekalska, Raphaël PB Jacquat, Quentin Peter, Walther C Traberg, Arvind Pujari, Akhila K Jayaram, Pavankumar Challa, Christopher G Taylor, Lize-Mari van der Linden, Titus Franzmann, Roisin M Owens, Simon Alberti, David Klenerman, Tuomas PJ Knowles
Feb 09, 2023
Attoliter protein nanogels from droplet nanofluidics for intracellular delivery.
Microscale hydrogels consisting of macromolecular networks in aqueous continuous phases have received increasing attention because of their potential use in tissue engineering, cell encapsulation and for the storage and release...
Direct digital sensing of protein biomarkers in solution.
Georg Krainer, Kadi L Saar, William E Arter, Timothy J Welsh, Magdalena A Czekalska, Raphaël P B Jacquat, Quentin Peter, Walther C Traberg, Arvind Pujari, Akhila K Jayaram, Pavankumar Challa, Christopher G Taylor, Lize-Mari van der Linden, Titus Franzmann, Roisin M Owens, Simon Alberti, David Klenerman, Tuomas P J Knowles
Mar 09, 2023
Published by: Nature Communications
A counterintuitive stereochemical outcome from a chelation-controlled vinylmetal aldehyde addition leads to the configurational reassignment of phormidolide A.
As part of our ongoing studies towards the total synthesis of phormidolide A (1), we explored the chelation-controlled vinylmetal addition of iodide 2 to aldehyde 3 to install the reported 17S configuration. While the...
Scalable integration of nano-, and microfluidics with hybrid two-photon lithography
Oliver Vanderpoorten, Quentin Peter, Pavan K. Challa, Ulrich F. Keyser, Jeremy Baumberg, Clemens F. Kaminski, Tuomas P. J. Knowles
Sep 08, 2020
Abstract: Nanofluidic devices have great potential for applications in areas ranging from renewable energy to human health. A crucial requirement for the successful operation of nanofluidic devices is the ability to interface...
Handling Imbalanced Data With Weighted Logistic Regression and Propensity Score Matching methods
The adoption of empirical methods for secondary data analysis has witnessed a significant surge in IS research. However, the secondary data is often incomplete, skewed, and imbalanced at best. Consequently, there is a growing...
Published by: IGI Global Scientific Publishing
Attoliter protein nanogels from droplet nanofluidics for intracellular delivery.
Microscale hydrogels consisting of macromolecular networks in aqueous continuous phases have received increasing attention because of their potential use in tissue engineering, cell encapsulation and for the storage and release...
Published by: Science advances
Enhanced Quality Factor Label-free Biosensing with Micro-Cantilevers Integrated into Microfluidic Systems.
Tadas Kartanas, Victor Ostanin, Pavan Kumar Challa, Ronan Daly, Jerome Charmet, Tuomas PJ Knowles
Jun 11, 2018
Microelectromechanical systems (MEMS) have enabled the development of a new generation of sensor platforms. Acoustic sensor operation in liquid, the native environment of biomolecules, causes, however, significant degradation of...
Low-Cost Microfabrication Tool Box.
Jérôme Charmet, Rui Rodrigues, Ender Yildirim, Pavan Kumar Challa, Benjamin Roberts, Robert Dallmann, Yudan Whulanza
Apr 14, 2020
Microsystems are key enabling technologies, with applications found in almost every industrial field, including in vitro diagnostic, energy harvesting, automotive, telecommunication, drug screening, etc. Microsystems, such as...
Low-Cost Microfabrication Tool Box
Microsystems are key enabling technologies, with applications found in almost every industrial field, including in vitro diagnostic, energy harvesting, automotive, telecommunication, drug screening, etc. Microsystems, such as...
Rapid two-dimensional characterisation of proteins in solution
Kadi L. Saar, Quentin Peter, Thomas Müller, Pavan K. Challa, Therese W. Herling, Tuomas P. J. Knowles
Jun 30, 2020
Abstract: Microfluidic platforms provide an excellent basis for working with heterogeneous samples and separating biomolecular components at high throughput, with high recovery rates and by using only very small sample volumes....
Biogenic Photo-Catalyst TiO2 Nanoparticles for Remediation of Environment Pollutants.
Boya Palajonnala Narasaiah, Pravallika Banoth, Angel Guillermo Bustamante Dominguez, Badal Kumar Mandal, Challa Kiran Kumar, Crispin HW Barnes, Luis De Los Santos Valladares, Pratap Kollu
Sep 07, 2022
This article reports a benign environmentally friendly fabrication method of titanium dioxide (TDO) nanoparticles (named TDO NPs3, TDO NPs5, and TDO NPs8) using aqueous extract of durva herb waste. This synthesis process avoids...
Real-Time Intrinsic Fluorescence Visualization and Sizing of Proteins and Protein Complexes in Microfluidic Devices.
Pavan Kumar Challa, Quentin Peter, Maya A Wright, Yuewen Zhang, Kadi L Saar, Jacqueline A Carozza, Justin LP Benesch, Tuomas PJ Knowles
Aug 18, 2020
Optical detection has become a convenient and scalable approach to read out information from microfluidic systems. For the study of many key biomolecules, however, including peptides and proteins, which have low fluorescence...
Rapid two-dimensional characterisation of proteins in solution.
Kadi L Saar, Quentin Peter, Thomas Müller, Pavan K Challa, Therese W Herling, Tuomas PJ Knowles
Apr 03, 2019
Microfluidic platforms provide an excellent basis for working with heterogeneous samples and separating biomolecular components at high throughput, with high recovery rates and by using only very small sample volumes. To date...
On-chip label-free protein analysis with downstream electrodes for direct removal of electrolysis products.
Kadi L Saar, Yingbo Zhang, Thomas Müller, Challa P Kumar, Sean Devenish, Andrew Lynn, Urszula Łapińska, Xiaoting Yang, Sara Linse, Tuomas PJ Knowles
Apr 16, 2018
The ability to apply highly controlled electric fields within microfluidic devices is valuable as a basis for preparative and analytical processes. A challenge encountered in the context of such approaches in conductive media...
Accelerating Reaction Rates of Biomolecules by Using Shear Stress in Artificial Capillary Systems.
Tuuli A Hakala, Emma V Yates, Pavan K Challa, Zenon Toprakcioglu, Karthik Nadendla, Dijana Matak-Vinkovic, Christopher M Dobson, Rodrigo Martínez, Francisco Corzana, Tuomas P J Knowles, Gonçalo J L Bernardes
Nov 04, 2021
Published by: Journal of the American Chemical Society
Accelerating Reaction Rates of Biomolecules by Using Shear Stress in Artificial Capillary Systems.
Tuuli A Hakala, Emma V Yates, Pavan K Challa, Zenon Toprakcioglu, Karthik Nadendla, Dijana Matak-Vinkovic, Christopher M Dobson, Rodrigo Martínez, Francisco Corzana, Tuomas PJ Knowles, Gonçalo JL Bernardes
Sep 16, 2021
Biomimetics is a design principle within chemistry, biology, and engineering, but chemistry biomimetic approaches have been generally limited to emulating nature's chemical toolkit while emulation of nature's physical toolkit...
Massively parallel C. elegans tracking provides multi-dimensional fingerprints for phenotypic discovery.
Michele Perni, Pavan K Challa, Julius B Kirkegaard, Ryan Limbocker, Mandy Koopman, Maarten C Hardenberg, Pietro Sormanni, Thomas Müller, Kadi L Saar, Lianne WY Roode, Johnny Habchi, Giulia Vecchi, Nilumi Fernando, Samuel Casford, Ellen AA Nollen, Michele Vendruscolo, Christopher M Dobson, Tuomas PJ Knowles
Jul 27, 2018
BACKGROUND: The nematode worm C. elegans is a model organism widely used for studies of genetics and of human disease. The health and fitness of the worms can be quantified in different ways, such as by measuring their bending...
Alzheimer’s disease Automation Drug Discovery neuroscience Parkinson’s disease Animals Behavior Animal Caenorhabditis elegans Data Interpretation Statistical Disease Models Animal Drug Evaluation Preclinical Machine Learning Neurodegenerative Diseases Optical Imaging pattern recognition Automated Phenotype Reproducibility of Results Software
A natural product inhibits the initiation of α-synuclein aggregation and suppresses its toxicity.
M Perni, C Galvagnion, A Maltsev, G Meisl, MBD Müller, PK Challa, JB Kirkegaard, P Flagmeier, SIA Cohen, R Cascella, SW Chen, R Limboker, P Sormanni, GT Heller, FA Aprile, N Cremades, C Cecchi, F Chiti, EAA Nollen, TPJ Knowles, M Vendruscolo, A Bax, M Zasloff, CM Dobson
Sep 02, 2019
Trodusquemine enhances Aβ42 aggregation but suppresses its toxicity by displacing oligomers from cell membranes.
Ryan Limbocker, Sean Chia, Francesco S Ruggeri, Michele Perni, Roberta Cascella, Gabriella T Heller, Georg Meisl, Benedetta Mannini, Johnny Habchi, Thomas CT Michaels, Pavan K Challa, Minkoo Ahn, Samuel T Casford, Nilumi Fernando, Catherine K Xu, Nina D Kloss, Samuel IA Cohen, Janet R Kumita, Cristina Cecchi, Michael Zasloff, Sara Linse, Tuomas PJ Knowles, Fabrizio Chiti, Michele Vendruscolo, Christopher M Dobson
Jan 17, 2019
Multistep Inhibition of α-Synuclein Aggregation and Toxicity in Vitro and in Vivo by Trodusquemine.
Michele Perni, Patrick Flagmeier, Ryan Limbocker, Roberta Cascella, Francesco A Aprile, Céline Galvagnion, Gabriella T Heller, Georg Meisl, Serene W Chen, Janet R Kumita, Pavan K Challa, Julius B Kirkegaard, Samuel IA Cohen, Benedetta Mannini, Denise Barbut, Ellen AA Nollen, Cristina Cecchi, Nunilo Cremades, Tuomas PJ Knowles, Fabrizio Chiti, Michael Zasloff, Michele Vendruscolo, Christopher M Dobson
Jul 18, 2019
The aggregation of α-synuclein, an intrinsically disordered protein that is highly abundant in neurons, is closely associated with the onset and progression of Parkinson's disease. We have shown previously that the aminosterol...
Comparative Studies in the A30P and A53T α-Synuclein C. elegans Strains to Investigate the Molecular Origins of Parkinson's Disease.
Michele Perni, Annemieke van der Goot, Ryan Limbocker, Tjakko J van Ham, Francesco A Aprile, Catherine K Xu, Patrick Flagmeier, Karen Thijssen, Pietro Sormanni, Giuliana Fusco, Serene W Chen, Pavan K Challa, Julius B Kirkegaard, Romain F Laine, Kai Yu Ma, Martin BD Müller, Tessa Sinnige, Janet R Kumita, Samuel IA Cohen, Renée Seinstra, Gabriele S Kaminski Schierle, Clemens F Kaminski, Denise Barbut, Alfonso De Simone, Tuomas PJ Knowles, Michael Zasloff, Ellen AA Nollen, Michele Vendruscolo, Christopher M Dobson
May 07, 2021
The aggregation of α-synuclein is a hallmark of Parkinson's disease (PD) and a variety of related neurological disorders. A number of mutations in this protein, including A30P and A53T, are associated with familial forms of the...
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