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Results: 87
Anti-D monoclonal antibodies from 23 human and rodent cell lines display diverse IgG Fc-glycosylation profiles that determine their clinical efficacy.
Belinda M Kumpel, Radka Saldova, Carolien A M Koeleman, Jodie L Abrahams, Agnes Hipgrave Ederveen, Kathryn L Armour, Natalia I Olovnikova, Gestur Vidarsson, Rick Kapur, Pauline M Rudd, Manfred Wuhrer
Mar 02, 2020
Anti-D immunoglobulin (Anti-D Ig) prophylaxis prevents haemolytic disease of the fetus and newborn. Monoclonal IgG anti-Ds (mAb-Ds) would enable unlimited supplies but have differed in efficacy in FcγRIIIa-mediated ADCC assays...
Published by: Scientific reports
Systematic Review
Chrissy Bolton, Yifan Chen, Rachel Hawthorne, Ianthe R. M. Schepel, Elinor Harriss, Silke C. Hofmann, Spencer Ellis, Alexander Clarke, Helena Wace, Blanca Martin, Joel Smith
Nov 26, 2020
Abstract: Background: Subacute cutaneous lupus erythematosus (SCLE) lacks consensus diagnostic criteria and the pathogenesis is poorly understood. There are increasing reports of SCLE induced by monoclonal antibodies (mAbs), but...
Monoclonal antibody targeting of fibroblast growth factor receptor 1c ameliorates obesity and glucose intolerance via central mechanisms.
Christopher J Lelliott, Andrea Ahnmark, Therese Admyre, Ingela Ahlstedt, Lorraine Irving, Feenagh Keyes, Laurel Patterson, Michael B Mumphrey, Mikael Bjursell, Tracy Gorman, Mohammad Bohlooly-Y, Andrew Buchanan, Paula Harrison, Tristan Vaughan, Hans-Rudolf Berthoud, Daniel Lindén
Feb 18, 2021
We have generated a novel monoclonal antibody targeting human FGFR1c (R1c mAb) that caused profound body weight and body fat loss in diet-induced obese mice due to decreased food intake (with energy expenditure unaltered), in...
Animals Antibodies Monoclonal Arcuate Nucleus of Hypothalamus Chemokine CCL2 Chemokine CCL7 Circumventricular Organs Eating Energy Metabolism Female Gene Expression Regulation Glucose Intolerance Humans Hypothalamus Leptin Mice Mice Knockout Mice Obese Mitogen-Activated Protein Kinases obesity Receptor Fibroblast Growth Factor Type 1 Receptor Melanocortin Type 4 Receptors Somatostatin Ribosomal Protein S6 Kinases 70-kDa Serum Response Factor Signal Transduction
Anti-D monoclonal antibodies from 23 human and rodent cell lines display diverse IgG Fc-glycosylation profiles that determine their clinical efficacy.
Belinda M Kumpel, Radka Saldova, Carolien AM Koeleman, Jodie L Abrahams, Agnes Hipgrave Ederveen, Kathryn L Armour, Natalia I Olovnikova, Gestur Vidarsson, Rick Kapur, Pauline M Rudd, Manfred Wuhrer
Jan 29, 2021
Anti-D immunoglobulin (Anti-D Ig) prophylaxis prevents haemolytic disease of the fetus and newborn. Monoclonal IgG anti-Ds (mAb-Ds) would enable unlimited supplies but have differed in efficacy in FcγRIIIa-mediated ADCC assays...
SARS-CoV-2 variant evasion of monoclonal antibodies based on in vitro studies.
MacGregor Cox, Thomas P Peacock, William T Harvey, Joseph Hughes, Derek W Wright, COVID-19 Genomics UK (COG-UK) Consortium, Brian J Willett, Emma Thomson, Ravindra K Gupta, Sharon J Peacock, David L Robertson, Alessandro M Carabelli
Nov 28, 2022
Monoclonal antibodies (mAbs) offer a treatment option for individuals with severe COVID-19 and are especially important in high-risk individuals where vaccination is not an option. Given the importance of understanding the...
A novel therapeutic antibody screening method using bacterial high-content imaging reveals functional antibody binding phenotypes of Escherichia coli ST131.
Mailis Maes, Zoe A Dyson, Sarah E Smith, David A Goulding, Catherine Ludden, Stephen Baker, Paul Kellam, Stephen T Reece, Gordon Dougan, Josefin Bartholdson Scott
Sep 21, 2020
The increase of antimicrobial resistance (AMR), and lack of new classes of licensed antimicrobials, have made alternative treatment options for AMR pathogens increasingly attractive. Recent studies have demonstrated...
Anti-Bacterial Agents Antibodies Monoclonal Humanized Drug Resistance Bacterial Escherichia coli Escherichia coli Infections Feasibility Studies High-Throughput Screening Assays Humans Interspersed Repetitive Sequences Microbial Sensitivity Tests Microscopy Electron O Antigens Phylogeny Polymorphism Single Nucleotide Virulence
Development of Inhibitors against Mycobacterium abscessus tRNA (m1G37) Methyltransferase (TrmD) Using Fragment-Based Approaches.
Andrew J Whitehouse, Sherine E Thomas, Karen P Brown, Alexander Fanourakis, Daniel S-H Chan, M Daben J Libardo, Vitor Mendes, Helena IM Boshoff, R Andres Floto, Chris Abell, Tom L Blundell, Anthony G Coyne
Jul 19, 2019
Mycobacterium abscessus (Mab) is a rapidly growing species of multidrug-resistant nontuberculous mycobacteria that has emerged as a growing threat to individuals with cystic fibrosis and other pre-existing chronic lung diseases....
Systematic Review
Chrissy Bolton, Yifan Chen, Rachel Hawthorne, Ianthe R. M. Schepel, Elinor Harriss, Silke C. Hofmann, Spencer Ellis, Alexander Clarke, Helena Wace, Blanca Martin, Joel Smith
Dec 22, 2020
Abstract: Background: Subacute cutaneous lupus erythematosus (SCLE) lacks consensus diagnostic criteria and the pathogenesis is poorly understood. There are increasing reports of SCLE induced by monoclonal antibodies (mAbs), but...
Rapid Isolation of Rare Isotype-Switched Hybridoma Variants
Stéphanie Charrin, Roberta Palmulli, Martine Billard, Denis Clay, Claude Boucheix, Guillaume Van Niel, Eric Rubinstein
Jul 04, 2020
CD63, a member of the tetraspanin superfamily, is used as a marker of late endosomes and lysosome-related organelles, as well as a marker of exosomes. Here, we selected rare isotype variants of TS63 by sorting hybridoma cells on...
Identification of immunostimulatory dendritic cells in the synovial effusions of patients with rheumatoid arthritis
Dendritic cells in the circulation are leukocytes that are rich in Ia antigens and that actively stimulate T cell replication. We have identified dendritic cells in the joint effusions of patients with rheumatoid arthritis. By...
Published by: Rockefeller University
A small number of anti-CD3 molecules on dendritic cells stimulate DNA synthesis in mouse T lymphocytes
Resting T cells enter cell cycle when challenged with anti-CD3 mAb and accessory cells that bear required Fc receptor (FcR). Presentation of anti-CD3 is though to be a model for antigens presented by accessory cells to the TCR...
Published by: Rockefeller University
In vitro model for predicting bioavailability of subcutaneously injected monoclonal antibodies
Hanne Kinnunen Bown, Catherine Bonn, Stefan T. Yohe, Daniela Bumbaca Yadav, Thomas W. Patapoff, Ann L. Daugherty, Randall J. Mrsny
Mar 10, 2018
Monoclonal antibodies (mAbs), which are now more frequently administered by subcutaneous (SC) injection rather than intravenously, have become a tremendously successful drug format across a wide range of therapeutic areas....
In vitro model for predicting bioavailability of subcutaneously injected monoclonal antibodies
Hanne Kinnunen Bown, Catherine Bonn, Stefan T. Yohe, Daniela Bumbaca Yadav, Thomas W. Patapoff, Ann L. Daugherty, Randall J. Mrsny
Mar 10, 2018
Monoclonal antibodies (mAbs), which are now more frequently administered by subcutaneous (SC) injection rather than intravenously, have become a tremendously successful drug format across a wide range of therapeutic areas....
Systematic Review
Chrissy Bolton, Yifan Chen, Rachel Hawthorne, Ianthe RM Schepel, Elinor Harriss, Silke C Hofmann, Spencer Ellis, Alexander Clarke, Helena Wace, Blanca Martin, Joel Smith
Sep 22, 2021
BACKGROUND: Subacute cutaneous lupus erythematosus (SCLE) lacks consensus diagnostic criteria and the pathogenesis is poorly understood. There are increasing reports of SCLE induced by monoclonal antibodies (mAbs), but there are...
Investigating the lowest threshold of vascular benefits from LDL cholesterol lowering with a PCSK9 mAb inhibitor (alirocumab) in healthy volunteers - a mechanistic physiological study (INTENSITY-LOW)
Michael S Kostapanos, Paul J Cacciottolo, Annette Hubsch, Holly Pavey, James Hurlock, Kaisa Maki-Petaja, Ian B Wilkinson, Joseph Cheriyan
Oct 15, 2019
Objective: Whether reducing low density lipoprotein cholesterol (LDL-C) is associated with cardiovascular benefits in low risk normocholesterolaemic subjects is unknown. The INTENSITY LOW [Investigating the lowest threshold of...
Investigating the lowest threshold of vascular benefits from LDL cholesterol lowering with a PCSK9 mAb inhibitor (alirocumab) in healthy volunteers - a mechanistic physiological study (INTENSITY-LOW)
Michael S Kostapanos, Paul J Cacciottolo, Annette Hubsch, Holly Pavey, James Hurlock, Kaisa Maki-Petaja, Ian B Wilkinson, Joseph Cheriyan
Dec 10, 2019
Objective: Whether reducing low density lipoprotein cholesterol (LDL-C) is associated with cardiovascular benefits in low risk normocholesterolaemic subjects is unknown. The INTENSITY LOW [Investigating the lowest threshold of...
Constrained invariant mass distributions in cascade decays
Christopher G Lester
Jan 25, 2010
Considering the cascade decay D → cC → cbB → cbaA in which D,C,B,A are
massive particles and c, b, a are massless particles, we determine for the shape of the
distribution of the invariant mass of the three massless particles...
An antigen-independent contact mechanism as an early step in T cell-proliferative responses to dendritic cells
Dendritic cells bearing antigen efficiently aggregate and stimulate antigen-specific T cells. We describe an experimental model in which an initial, apparently antigen-independent binding step is followed by ligation of the TCR....
Published by: Rockefeller University
Characterization of 7A7, an anti-mouse EGFR monoclonal antibody proposed to be the mouse equivalent of cetuximab.
Xuzhi He, Jazmina L Cruz, Shannon Joseph, Nicola Pett, Hui Yi Chew, Zewen K Tuong, Satomi Okano, Gabrielle Kelly, Margaret Veitch, Fiona Simpson, James W Wells
Aug 28, 2019
The Epidermal Growth Factor Receptor (EGFR) is selectively expressed on the surface of numerous tumours, such as non-small cell lung, ovarian, colorectal and head and neck carcinomas. EGFR has therefore become a target for...
Dissecting the stability of Atezolizumab with renewable amino acid-based ionic liquids
Bayan Alkhawaja, Faisal Al-Akayleh, Zaid Al-Rubaye, Ghayda' AlDabet, Muna Bustami, Maisa’a Smairat, Ahmed S.A.A. Agha, Jehad Nasereddin, Nidal Qinna, Andreas Michael, Andrew Watts
Jun 30, 2024
Monoclonal antibodies (mAbs) have revolutionised the biopharmaceutical market. Being proteinaceous, mAbs are prone to chemical and physical instabilities. Various approaches were attempted to stabilise proteins against...
Dissecting the stability of Atezolizumab with renewable amino acid-based ionic liquids
Bayan Alkhawaja, Faisal Al-Akayleh, Zaid Al-Rubaye, Ghayda' AlDabet, Muna Bustami, Maisa’a Smairat, Ahmed S.A.A. Agha, Jehad Nasereddin, Nidal Qinna, Andreas Michael, Andrew Watts
Jun 30, 2024
Monoclonal antibodies (mAbs) have revolutionised the biopharmaceutical market. Being proteinaceous, mAbs are prone to chemical and physical instabilities. Various approaches were attempted to stabilise proteins against...
Evaluation of Reactivity of Monoclonal Antibodies Against Omp25 of Brucella spp.
Xin Yang, Zuoping He, Guoxia Zhang, Jinhui Lu, Hui Zhang, Hui Ren, Yanjun Tian, Heng Yang, Chuangfu Chen, Linhai Li, Yongshui Fu, Jean-Pierre Allain, Chengyao Li, Wenjing Wang
May 05, 2020
Brucellosis is a serious zoonosis occurring mainly in developing countries, and its diagnosis is largely dependent on serologic detection and bacterial culture. In this study, we developed the murine monoclonal antibodies (mAbs)...
Recombinant human monoclonal HLA antibodies of different IgG subclasses recognising the same epitope
Cynthia SM Kramer, Marry EI Franke-van Dijk, Ashley J Priddey, Tamás Pongrácz, Elena Gnudi, Helena Car, Gonca E Karahan, Els van Beelen, Chalana CC Zilvold-van den Oever, Hendrik J Rademaker, Noortje de Haan, Manfred Wuhrer, Vasilis Kosmoliaptsis, Paul WHI Parren, Arend Mulder, Dave L Roelen, Frans HJ Claas, Sebastiaan Heidt
Oct 29, 2019
In the field of transplantation, the humoural immune response against mismatched HLA antigens of the donor is associated with inferior graft survival, but not in every patient. Donor-specific HLA antibodies (DSA) of different...
Epidermal langerhans cells rapidly capture and present antigens from C-type lectin-targeting antibodies deposited in the dermis
Antigen-presenting cells can capture antigens that are deposited in the skin, including vaccines given subcutaneously. These include different dendritic cells (DCs) such as epidermal Langerhans cells (LCs), dermal DCs, and...
Monoclonal antibody Antigen Presentation dendritic cell Dermis epidermis langerhans cell T-Lymphocytes
Published by: Rockefeller University
Dendritic cells are the principal cells in mouse spleen bearing immunogenic fragments of foreign proteins
We monitored the APC function of cells taken from the spleen and peritoneal cavity of mice that had been given protein antigens via the intravenous or intraperitoneal routes. Using the mAb 33D1 and N418 to negatively and...
Published by: Rockefeller University
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