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Advanced fluorescence imaging of in situ protein aggregation.
The aggregation of intrinsically disordered proteins is a hallmark of neurodegenerative diseases, such as Alzheimer's, Parkinson's and Huntington's disease. Although we currently have a good molecular level understanding on how...
Machine learning assisted interferometric structured illumination microscopy for dynamic biological imaging.
Biophotonics of Native Silk Fibrils.
Machine learning assisted interferometric structured illumination microscopy for dynamic biological imaging.
Edward N Ward, Lisa Hecker, Charles N Christensen, Jacob R Lamb, Meng Lu, Luca Mascheroni, Chyi Wei Chung, Anna Wang, Christopher J Rowlands, Gabriele S Kaminski Schierle, Clemens F Kaminski
Jan 22, 2023
Structured Illumination Microscopy, SIM, is one of the most powerful optical imaging methods available to visualize biological environments at subcellular resolution. Its limitations stem from a difficulty of imaging in multiple...
C-terminal calcium binding of α-synuclein modulates synaptic vesicle interaction.
Janin Lautenschläger, Amberley D Stephens, Giuliana Fusco, Florian Ströhl, Nathan Curry, Maria Zacharopoulou, Claire H Michel, Romain Laine, Nadezhda Nespovitaya, Marcus Fantham, Dorothea Pinotsi, Wagner Zago, Paul Fraser, Anurag Tandon, Peter St George-Hyslop, Eric Rees, Jonathan J Phillips, Alfonso De Simone, Clemens F Kaminski, Gabriele S Kaminski Schierle
Jul 31, 2018
Alpha-synuclein is known to bind to small unilamellar vesicles (SUVs) via its N terminus, which forms an amphipathic alpha-helix upon membrane interaction. Here we show that calcium binds to the C terminus of alpha-synuclein...
Nano-vehicles give new lease of life to existing antimicrobials.
Antibiotic resistance has become one of the greatest challenges for modern medicine, and new approaches for the treatment of bacterial infections are urgently needed to avoid widespread vulnerability again to infections that...
ML-SIM
Structured illumination microscopy (SIM) has become an important technique for
optical super-resolution imaging because it allows a doubling of image resolution at speeds
compatible with live-cell imaging. However, the...
Calcium imaging analysis - how far have we come?
Techniques for calcium imaging were first demonstrated in the mid-1970s, whilst tools to analyse these markers of cellular activity are still being developed and improved today. For image analysis, custom tools were developed...
classification Neural Networks Quantification denoising calcium imaging Machine Learning motion correction
Published by: F1000Research
A label-free, quantitative assay of amyloid fibril growth based on intrinsic fluorescence.
Dorothea Pinotsi, Alexander K Buell, Christopher M Dobson, Gabriele S Kaminski Schierle, Clemens F Kaminski
Feb 12, 2020
Kinetic assay of seeded growth: The graph shows the variation in intrinsic fluorescence intensity of amyloid fibrils. Fluorescence increases during the seeded aggregation of α-synuclein seeds with α-synuclein monomeric protein...
Analysis of the native structure, stability and aggregation of biotinylated human lysozyme.
Minkoo Ahn, Erwin De Genst, Gabriele S Kaminski Schierle, Miklos Erdelyi, Clemens F Kaminski, Christopher M Dobson, Janet R Kumita
Feb 12, 2020
Fibril formation by mutational variants of human lysozyme is associated with a fatal form of hereditary non-neuropathic systemic amyloidosis. Defining the mechanistic details of lysozyme aggregation is of crucial importance for...
Design of a Functionalized Metal-Organic Framework System for Enhanced Targeted Delivery to Mitochondria.
Salame Haddad, Isabel Abánades Lázaro, Marcus Fantham, Ajay Mishra, Joaquin Silvestre-Albero, Johannes W M Osterrieth, Gabriele S Kaminski Schierle, Clemens F Kaminski, Ross S Forgan, David Fairen-Jimenez
Apr 17, 2020
Mitochondria play a key role in oncogenesis and constitute one of the most important targets for cancer treatments. Although the most effective way to deliver drugs to mitochondria is by covalently linking them to a lipophilic...
Published by: Journal of the American Chemical Society
Structural basis of synaptic vesicle assembly promoted by α-synuclein.
Giuliana Fusco, Tillmann Pape, Amberley D Stephens, Pierre Mahou, Ana Rita Costa, Clemens F Kaminski, Gabriele S Kaminski Schierle, Michele Vendruscolo, Gianluigi Veglia, Christopher M Dobson, Alfonso De Simone
Mar 04, 2020
α-synuclein (αS) is an intrinsically disordered protein whose fibrillar aggregates are the major constituents of Lewy bodies in Parkinson's disease. Although the specific function of αS is still unclear, a general consensus is...
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